This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases.
DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair.
This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization.
It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease.
Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks.
Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemisty.
Other potential application, though not tested is Direct Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: Formalin fixed and paraffin embedded tissues Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes human WRN.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 787-816 in the centrall region of human WRN.
