PRDM2 is a 250-kD nuclear protein containing 8 zinc finger motifs.
It contains an RB-binding motif that is related to that of the adenovirus E1A oncoprotein; RIZ also shares an antigenic epitope with the C terminus of E1A.
It is expressed in human retinoblastoma cells and at low levels in all other human cell lines examined.
Using fluorescence in situ hybridization, the PRDM2 gene has been mapped to 1p36.
PRDM2 may be a candidate target of 1p36 alterations that commonly occur in neuroendocrine, breast, liver, colon, skin, and lymphoid tumors.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation, ELISA
Recommended Dilution:ELISA: 1:1,000Western Blot1:100-500Immunohistochemistry: 1:100-500Immunoprecipitation: 1 ug Pab/100 ug cell lysate
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Purified recombinant GST fusion protein encoding a portion within aa 245-573 of rat Riz1. This antibody also recognizes the isoform beta (Riz2) of PRDM2 (185 kDa).
