84-1690-82　［取扱停止］PRKAA2 (Thr172), ID (PRKAA2, AMPK, AMPK2, 5'-AMP-activated protein kinase catalytic subunit alpha-2, Acetyl-CoA carboxylase kinase, Hydroxymethylglutaryl-CoA reductase kinase) (Biotin) 200ul　040436-Biotin

特徴

• The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK).
• AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits.
• AMPK is an important energy-sensing enzyme that monitors cellular energy status.
• In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol.
• Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry (Not Tested) and ELISA.
• Other applications not tested.
• Recommended Dilutions:Immunohistochemistry: Formalin-fixed, paraffin-embedded sections Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.