84-1689-46　［取扱停止］HLA-B, ID (HLA-B, HLAB, HLA class I histocompatibility antigen, B-7 alpha chain, MHC class I antigen B*7) (Biotin) 200ul　036616-Biotin

特徴

• HLA-B belongs to the HLA class I heavy chain paralogues.
• This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin).
• The heavy chain is anchored in the membrane.
• Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen.
• They are expressed in nearly all cells.
• The heavy chain is approximately 45 kDa and its gene contains 8 exons.
• Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail.
• Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule.
• Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation.
• Hundreds of HLA-B alleles have been described.
• Applications:Suitable for use in Western Blot, ELISA
• Recommended Dilution:ELISA: 1:1,000Western Blot: 1:100-500
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.