Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems.
The HA tag (YPYDVPDYAYPYDVPDYA) is an established example.
Our anti-HA polyclonal antibody provides a simple solution to detect the expression of an HA-tagged protein in cells.
Applications:Suitable for use in Western Blot, Immunofluorescence, ChIP and ELISA.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein A/G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes Hemagglutinin Tag.
Isotype：IgG
Calc Applications Abbrev：E IF WB
Immunogen：KLH conjugated synthetic peptide encoding HA tag (CYPYDVPDYAYPYDVPDYA).
