84-1685-18　［取扱停止］UHRF1, ID (E3 Ubiquitin-protein Ligase UHRF1, Ubiquitin-like PHD and RING Finger Domain-containing Protein 1, Ubiquitin-like-containing PHD and RING Finger Domains Protein 1, Inverted CCAAT box-binding Protein of 90kD, Transcription Factor ICBP90, Nuclear Zinc Finger Protein Np95, HuNp95, RING Finger Protein 106, Nuclear Protein 95, ICBP90, NP95, RNF106) (Biotin) 200ul　U1008-01-Biotin

特徴

• UHRF1 is a member of a subfamily of RING-finger type E3 ubiquitin ligases.
• The protein binds to specific DNA sequences, and recruits a histone acetylase to regulate gene expression.
• Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle.
• It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:1,000Western Blot: 1:100-1:500Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.