SMAD3, a receptor regulated SMAD (R-SMAD) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase.
SMAD3 is estimated to account for at least 80% of all TGF-beta-mediated response.
Activated type I receptor phosphorylates receptor-activated SMADS (RSMADS) at their c-terminal two extreme serines in the SSXS motif.
The phosphorylated R-SMAD translocate into nucleus, where they regulate transcription of target genes.
SMAD3 signal transduction appears to be important in the rgulation of muscle-specific genes.
Loss of SMAD3 is a feature of pediatric T-cell lymphoblastic leukemia, while upregulation of SMAD3 may be responsible for TGFB hyperresponsiveness observed in scleroderma.
Applications:Suitable for use in ELISA, Dot Blot and Immunofluorescence.
Other applications not tested.
Recommended Dilution:ELISA: 1:1,000 Dot Blot: 1:500Immunofluorescence: 1:10-1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes human SMAD3 when phosphorylated at Ser208.
Isotype：IgG
Calc Applications Abbrev：DB E IF
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser208 of human SMAD3 (KLH).
