PPP6C belongs to the PPP phosphatase family, PP-V subfamily.
Reversible phosphorylation of proteins on serine and threonine residues is an important biochemical event that regulates a broad variety of intracellular processes.
The phosphorylation state is determined by the well-controlled balance of activities of serine/threonine-specific protein kinases and protein phosphatases, including PPP6C.
Expression levels are highest in testis, heart, and skeletal muscle and lowest in placenta, lung, and kidney.
PPP6C can complement mutations in the S.
cerevisiae Sit4 and S.
pombe ppe1 genes, indicating that PPP6C is the functional homolog of yeast Sit4p and ppe1.
Since Sit4p is required for the G1 to S transition of the cell cycle and ppe1 is involved in cell shape control and mitotic division, it has been suggested that PPP6C functions in cell cycle regulation.
Applications:Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:1,000Western Blot: 1:100-1:500Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes human PPP6C.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide selected from the N-terminal region of human PPP6C (KLH).
