Phosphatidylserine decarboxylases catalyze the formation of phosphatidylethanolamine (PE) by decarboxylation of phosphatidylserine (PS).
Type I PSDs, such as PISD, are targeted to the inner mitochondrial membrane by an N-terminal targeting sequence.
PISD also contains a conserved LGST motif that functions as an autocatalytic cleavage site where the proenzyme is split into mature alpha and beta subunits
Applications:Suitable for use in ELISA, Immunohistochemistry, Flow Cytometry (Not Tested) and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:1,000Western Blot: 1:100-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:200ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by Protein A and peptide affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity:Recognizes human PISD. Species Crossreactivity: mouse.
Isotype:IgG
Calc Applications Abbrev:E IHC WB
Calc Crossreactivity:Hu Mo
Immunogen:KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 223-250 in the C-terminal region of human PISD, UniProt Accession #Q9UG56; NP_055153. Cellular Localization: Mitochondrion