NEK9, a member of the NIMA subfamily of Ser/Thr protein kinases, is a pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation.
It phosphorylates different histones (serine and threonine residues on H3), myelin basic protein, beta-casein (serine residues), and BICD2.
NEK9 is activated during mitosis by intramolecular autophosphorylation.
Activity and autophosphorylation is activated by manganese >> magnesium ions, and is sensitive to increasing concentration of detergents.
This protein is not cell-cycle regulated but activity is higher in G0-arrested cells.
NEK is part of a homodimer that binds to Ran GTPase, and exhibits a greater affinity for Ran-GDP over Ran-GTP.
Interaction is also noted with NEK6 and NEK7 family members.
This cytoplasmic protein is most abundant in heart, liver, kidney and testis, and is also expressed in smooth muscle cells and fibroblasts.
Expression varies mildly across the cell cycle, with highest expression observed in G1 and stationary-phase cells.
Applications:Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:1,000Western Blot: 1:100-1:500Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes human NEK9. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide selected from the C-terminal region of human NEK9 (KLH).
