The myotubularin (MTM) family constitutes one of the largest and most highly conserved protein-tyrosine phosphatase (PTP) subfamilies.
Myotubularins, contain the consensus active site of tyrosine phosphatases but otherwise shows no homology to other phosphatases.
PTP's usually act on substrates containing only phosphotyrosine sites, but myotubularins were shown to act on both phosphotyrosine and phosphoserine (dual specific).
The enzymatic activity of myotubularins had not been demonstrated previously because it lacks catalytically active residues in tyrosine phosphatase/dual-specific phosphatase active site.
The active site is however sufficiently preserved to bind phosphorylated substrates, and may protect from phosphatases.
It was reported that interaction of myotubularin family members makes one of them catalytically active.
MTMR5 can interact with MTMR2 and regulates the enzymatic activity and the subcellular localization of MTMR2.
MTMR5 inhibits myoblast differentiation in vitro and induces oncogenic transformation in fibroblasts.Other suggested roles for MTMR5 are in spermatogenesis and germ cell differentiation.
Binding partners for MTMR5 include the SET domain of MLL/HRX and SUV39H1.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:1,000 Western Blot: 1:100-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes human MTMR5. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide selected from the C-terminal region of human MTMR5 (KLH).
