84-1678-63　［取扱停止］Inositol 1,4,5-trisphosphate 3-kinase A, CT (Inositol-trisphosphate 3-kinase A, InsP 3-kinase A, IP3 3-kinase A, IP3K A, IP3KA, IP3-3KA, ITPKA) (Biotin) 200ul　I7655-65C-Biotin

特徴

• ITPKA regulates inositol phosphate metabolism by phosphorylation of second messenger inositol 1,4,5-trisphosphate to Ins(1,3,4,5)P4.
• The activity of the inositol 1,4,5-trisphosphate 3-kinase is responsible for regulating the levels of a large number of inositol polyphosphates that are important in cellular signaling.
• Both calcium/calmodulin and protein phosphorylation mechanisms control its activity.
• It is also a substrate for the cyclic AMP-dependent protein kinase, calcium/calmodulin- dependent protein kinase II, and protein kinase C in vitro.
• ITPKA and ITPKB are 68% identical in the C-terminus region.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:1,000 Western Blot: 1:50-1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.