84-1678-55　［取扱停止］ILK (Ser436) (Integrin-linked Protein Kinase, ILK-1, ILK-2, 59kD Serine/Threonine-protein Kinase, p59ILK, ILK1, ILK2) (Biotin) 200ul　I5005-01H-Biotin

特徴

• Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins.
• Integrin-linked kinase (ILK) is an ankyrin repeat containing 51kD receptor-proximate serine-threonine kinase (1), with a reported migration rate of 59K.
• This 451aa protein interacts with the cytoplasmic domain of the beta-1 integrin subunit and contains sequence motifs found in pleckstrin homology domains capable of interacting with phosphoinositide lipids.
• ILK is an upstream regulator of Pi(3)K dependent activation of protein kinase B (PKB/AKT) and inhibition of glycogen synthase kinase 3 (GSK-3).
• ILK2 expression is associated with mediation of cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells.
• ILK2 is overexpressed in some highly invasive tumor cell lines.
• Applications:Suitable for use in ELISA and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:1,000Immunohistochemistry: 1:10-1:50Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.