Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities.
Nitric oxide is synthesized from L-arginine by nitric oxide synthases.
Applications:Suitable for use in ELISA and Dot Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:1,000 Dot Blot: 1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:200ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by Protein A affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity:Recognizes human eNos when phosphorylated at Ser1177.
Isotype:IgG
Calc Applications Abbrev:DB E
Calc Crossreactivity:Hu
Immunogen:Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser1177 of human eNos (KLH).