TAOK1 phosphorylated Mark on a threonine within its activation loop.
In brain, TAOK1 also phosphorylated a fraction of Mark on a nearby serine, and this phosphorylation inhibited Mark activity.
In cells, TAOK1 activity enhanced microtubule dynamics through activation of Mark and led to phosphorylation and detachment of microtubule-associated proteins from microtubules.
TAOK1 also activated JNK in vitro.
Overexpression of TAOK1 in a human nonsmall cell lung carcinoma cell line induced apoptotic morphologic changes, including cell contraction, membrane blebbing, and apoptotic body formation.
Apoptotic stimuli increased the catalytic activity of endogenous TAOK1 and JNK, and dominant-negative JNK or JNK inhibition blocked the apoptotic morphologic responses to TAOK1.
TAOK1 also stimulated cleavage and activation of ROCK1 by caspases, leading to cell contraction and membrane blebbing.
TAOK1 was itself a substrate for caspase-3.
TAOK1 is indeed involved in the execution phase of apoptosis.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:1,000Western Blot: 1:100-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes human TAOK1.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide selected from the center region of human TAOK1 (KLH).
