84-1672-10　［取扱停止］Stat 5a, phosphorylated (Ser726) (Signal Transducer And Activator Of Transcription 5A, STAT5A, STAT5) (Biotin) 200ul　S7972-10B-Biotin

特徴

• STAT5a is a member of the STAT family of transcription factors.
• In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators.
• This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones.
• Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis.
• The mouse counterpart of this protein is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this protein in cells.
• Applications:Suitable for use in ELISA, Western Blot, Dot Blot, and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:1,000Western Blot: 1:100-1:500Immunohistochemistry: 1:50-1:100Dot Blot: 1:500Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.