84-1671-76　［取扱停止］Separase, phosphorylated (Ser748) (ESPL1, Separin, Caspase-like Protein ESPL1, Extra Spindle Poles-like 1 Protein, ESP1, KIAA0165) (Biotin) 200ul　S0952-01F-Biotin

特徴

• ESPL1 is a caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase.
• During most of the cell cycle, it is inactivated by different mechanisms.
• ESPL1 is regulated by at least two independent mechanisms.
• First, it is inactivated via its interaction with securin/PTTG1, which probably covers its active site.
• The association with PTTG1 is not only inhibitory, since PTTG1 is also required for activating it, the enzyme being inactive in cells in which PTTG1 is absent.
• PTTG1 degradation at anaphase, liberates it and triggers RAD21 cleavage.
• Second, phosphorylation at Ser-1126 inactivates it.
• The complete phosphorylation during mitosis, is removed when cells undergo anaphase.
• Activation of the enzyme at the metaphase-anaphase transition probably requires the removal of both securin and inhibitory phosphate.
• Applications:Suitable for use in ELISA and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:1,000Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.