84-1671-63　［取扱停止］CDC25B, phosphorylated (Ser187) (M-phase Inducer Phosphatase 2, Dual Specificity Phosphatase Cdc25B, CDC25HU2) (Biotin) 200ul　C2560-04E-Biotin

特徴

• CDC25B is a member of the CDC25 family of phosphatases.
• CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis.
• CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals.
• The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2.
• CDC25B has oncogenic properties, although its role in tumor formation has not been determined.
• Multiple transcript variants for this gene exist.
• Applications:Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:1,000Western Blot: 1:100-1:500Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.