Sulfotransferase (SULT) enzymes catalyze the sulfate conjugation of many drugs, xenobiotic compounds, hormones, and neurotransmitters.
3'-phosphoadenosine 5'-phosphosulfate (PAPS) synthase (PAPSS) catalyzes the biosynthesis of PAPS which serves as the universal sulfonate donor compound for all sulfotransferase reactions.
In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 (603005).
Bifunctional PAPSS1 is comprised of an N-terminal APS kinase domain, and a C-terminal ATP sulfurylase domain.
Full-length protein has significantly less APS kinase activity than the N-terminal fragment, suggesting that the C-terminal domain exerts a regulatory role on the N-terminal APS kinase activity.
In humans there are two major isoforms: PAPSS1 and PAPSS2.
In brain and skin PAPSS1 is the major isoform, whereas in liver, cartilage and adrenal glands PAPSS2 isoform expression dominates.
The predicted 623aa protein is 98% identical to mouse PAPS synthase.
The N-terminal 268aa region of human PAPS synthase resembles APS kinases from other organisms and contains 3 conserved nucleotide-binding motifs.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:1,000 Western Blot: 1:100-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes human PAPSS1.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide selected from the C-terminal region of human PAPSS1 (KLH).
