Cytosolic sulfotransferases (STs or SULTs) catalyze the sulfate conjugation of many drugs, xenobiotic compounds, hormones, and neurotransmitters.
Her et al.
(1998) identified an EST with homology to ST enzymes.
By PCR with human placental and prostate cDNA as template, they isolated 2 alternatively spliced cDNAs, identical throughout most of their sequences, but having different 5-prime ends.
The shorter cDNA, SULT2B1a, encodes a protein of 350 amino acids; the longer cDNA, SULT2B1b, encodes a 365aa protein.
Genomic PCR analysis revealed that the gene encoding both cDNAs, SULT2B1, contains 7 exons, with 2 alternative first exons being used to generate SULT2B1a and SULT2B1b.
Northern blot analysis revealed that the SULT2B1 gene is expressed as a 1.4-kb transcript predominantly in prostate, placenta, and trachea.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:1,000 Western Blot: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes human SULT2B1, isoforms a and b.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide selected from the N-terminal region of human SULT2B1a/b (KLH).
