CBX4 is a member of Drosophila Polycomb group gene family.
The polycomb group (PcG) genes are essential for maintenance of proper expression patterns of developmental master regulators; changes in expression pf PcG proteins have been associated with cancer.
CBX4 is a part of the cellular memory system responsible for the inheritance of gene activity by progeny cells.
It participates in maintaining the transcriptionally repressive state of genes.
CBX4 is part of acomplex that acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
CBX4 is an E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I.
Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (FFPE): 1:50-1:100Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes human CBX4. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 80-110 in the N-terminal region of human CBX4.
