Myb is a transcriptional activator; DNA-binding protein that recognizes the sequence 5'-YAAC[GT]G-3'.
It participates in the control of proliferation and differentiation of hematopoietic progenitor cells.
Myb is a transcription factor related to Myc, and is expressed predominantly in haematopoietic cells and immature gastroepithelial cells.
TRAF7 stimulates the sumoylation of Myb at Lys-523 and Lys-499, which are the same sites as those used for PIASy-induced sumoylation.
A correlation has been established between reduction in sumoylation of Myb and increase in transcriptional activation.
Negative influence on transactivation properties by the negative regulatory domain region of c-Myb depends on upon sumoylation.
Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:1000Immunohistochemistry (Paraffin): 1:50-1:100Western Blot: 1:100-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes human MYB. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide PLLKKIKQEVESPTCA containing a predicted sumoylation site from the C-terminal region of human MYB (KLH).
