84-1665-89　［取扱停止］Dnmt3L (DNA (Cytosine-5)-methyltransferase 3-like) (Biotin) 200ul　D3931-73M-Biotin

特徴

• mDnmt3L (DNA methyltransferase 3L) does not appear to be catalytically active due to loss of the critical active site residues.
• It may function not directly as a DNA methyltransferase but rather as a regulator of methylation at imprinted loci.
• It is required specifically for the establishment of genomic imprints but is dispensable for their propagation.
• It is essential for the de novo methylation of single-copy DNA sequences.
• The protein is well-express in testis, thymus, ovary, and heart.
• mDnmt3L, which belongs to the 5-cytosine methyltransferase family, contains 1 ADD-type zinc finger.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:1,000 Western Blot: 1:100-1:500Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.