This gene encodes the basic form of complement factor 4, part of the classical activation pathway.
The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion.
The trimer provides a surface for interaction between the antigen-antibody complex and other complement components.
The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation.
Deficiency of this protein is associated with systemic lupus erythematosus.
This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6.
Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene.
In addition, this gene exists as a long form and a short form due to the presence or absence of a 6.4 kb endogenous HERV-K retrovirus in intron 9.
Applications:Suitable for use in Western Blot and FLISA.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide and a stabilizer. Labeled with Allophycocyanin (APC).
Specificity：Recognizes human C4B.
Isotype：IgG
Calc Applications Abbrev：FLISA WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 655-684 in the central region of human C4B.
