84-1577-39　［取扱停止］Smad2 (Mothers Against Decapentaplegic Homolog 2, Mothers Against DPP Homolog 2, MAD Homolog 2, Mad-related Protein 2, hMAD-2, SMAD Family Member 2, SMAD 2, hSMAD2, JV18-1, MADR2, MADH2) (APC) 200ul　S1014-52P-APC

特徴

• The protein belongs to the SMAD, a family of proteins similar to the proteins of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C.
• elegans gene Sma.
• SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways.
• This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation.
• This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein.
• In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors.
• The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4.
• The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors.
• This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin.
• Applications:Suitable for use in FLISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:FLISA: 1:1,000Western Blot: 1:50-1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: APC conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.