This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases.
This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein.
The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation.
Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines.
Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized.
Applications:Suitable for use in FLISA and Dot Blot.
Other applications not tested.
Recommended Dilution:FLISA: 1:1,000 Dot Blot: 1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide and a stabilizer. Labeled with Allophycocyanin (APC).
Specificity：Recognizes human RPS6KB1 when phosphorylated at Ser418.
Isotype：IgG
Calc Applications Abbrev：DB FLISA WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser418 of human RPS6KB1 (KLH).
