84-1576-85　［取扱停止］RAI17, CT (Zinc Finger MIZ Domain-containing Protein 1, Retinoic Acid-induced Protein 17, PIAS-like Protein Zimp10, ZMIZ1, KIAA1224, ZIMP10) (APC) 200ul　R1690-17-APC

特徴

• Retinoic acid plays a critical role in development, cellular growth, and differentiation and induces the expression of a variety of genes.
• RAI17 expression is induced by retinoic acid and is predominantly expressed in heart, brain and ovaries.
• Within brain, highest expression is in amygdala.
• The deduced 1,067aa protein contains an MSX-interacting zinc finger (MIZ) domain, a nuclear localization signal sequence, and 2 proline-rich regions.
• A strong intrinsic transactivation domain is identified in the C-terminal proline-rich region.
• RAI17 expression is detected in various cancer cell lines.
• RAI17 colocalizes with endogenous androgen receptor (AR) in the nuclei of prostate epithelial cells from human tissue samples.
• In human prostate cancer cells, RAI17 increases the transcriptional activity of AR.
• Studies using sumoylation-deficient AR mutants suggest that the increase of AR activity by RAI17 is dependent upon receptor sumoylation.
• Applications:Suitable for use in FLISA, Western Blot, and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:FLISA: 1:1,000Western Blot: 1:100-1:500Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: APC conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.