Phosphatidylinositolpolyphosphates (PtdInsPs) are centrally involved in many biologic processes, ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis.
PI4KII phosphorylates PtdIns at the D-4 position, an essential step in the biosynthesis of PtdInsPs.
PI4K II is activated by detergent and inhibited by adenosine.
Overexpression of PI4KII in COS-7 cells increases synthesis of PtdIns4P.
Some cells overexpressing PI4KII have scattered or no perinuclear Golgi.
Knockdown of PI4KII by RNA interference (RNAi) does not disrupt the Golgi, and some cells show expanded Golgi.
RNAi reduces the Golgi level of PtdIns4P and blocks the association between AP1 and the trans-Golgi network.
PI4KII RNAi had little effect on intra-Golgi trafficking, but it inhibited export to plasma membrane export by 35%.
It has been proposed that PI4KII generates PtdIns4P-rich domains within the Golgi that specify docking of the AP1 coat machinery.
Applications:Suitable for use in FLISA, Western Blot, Immunofluorescence, and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:FLISA: 1:1000Western Blot: 1:100-1:500Immunohistochemistry: 1:50-1:100Immunofluorescence: 1:10-1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide and a stabilizer. Labeled with Allophycocyanin (APC).
Specificity：Recognizes human PI4K2A.
Isotype：IgG
Calc Applications Abbrev：FLISA IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to a region near a portion within aa1-30 at the N-terminal of human PI4K2A, conjugated to KLH
