Stimulation of cells with cytokines initiates a signal transduction cascade involving cytokine receptors, Janus kinases (JAKs) and signal transducers and activators of transcription (STATs).
STAM for 'signal-transducing adaptor molecule, induced after stimulation of cells with cytokine IL2, is a component of signal transduction downstream of JAK3.1 Human STAM cDNA cloned from a T-cell cDNA library encodes a 540aa protein precipitated by anti-phosphotyrosine.
Northern blot analysis indicates that STAM is expressed as a 2.9-kb message in a wide variety of tissue and cell types.
The STAM sequence contains a Src-homology 3 (SH3) domain and an immunoreceptor tyrosine-based activation motif (ITAM).
It has been suggested that STAM acts as an adaptor molecule in signal transduction pathways from cytokine receptors.
Applications:Suitable for use in FLISA, Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Immunohistochemistry: Formalin-fixed, paraffin-embedded sectionsOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A/G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide and a stabilizer. Labeled with Allophycocyanin (APC).
Specificity：Recognizes human STAM.
Isotype：IgG
Calc Applications Abbrev：FLISA IHC WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 326-356 in the C-terminal region of human STAM. Species Sequence Homology: mouse
