SIGLEC9 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells.
It preferentially binds to alpha-2,3- or alpha-2,6-linked sialic acid.
The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
This protein is expressed by peripheral blood leukocytes (neutrophils and monocytes but not eosinophils).
It is found in liver, fetal liver, bone marrow, placenta, spleen and in lower levels in skeletal muscle, fetal brain, stomach, lung, thymus, prostate, brain, mammary, adrenal gland, colon, trachea, cerebellum, testis, small intestine and spinal cordon.
SIGLEC9 contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM).
This motif is involved in modulation of cellular responses.
The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
Applications:Suitable for use in FLISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:FLISA: 1:1,000Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide and a stabilizer. Labeled with Allophycocyanin (APC).
Specificity：Recognizes human SIGLEC9.
Isotype：IgG
Calc Applications Abbrev：FLISA IHC
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide selected from the N-terminal region of human SIGLEC9 (KLH).
