SCYL1 forms multimers following transfection into COS-7 cells.
SCYL1 forms a 300-kD trimer using crosslinking reagents.
Biochemical analysis revealed no phosphorylation or autophosphorylation activity.The 707-amino acid SCYL1 variant, variant 2, localized to centrosomes during mitosis.
During interphase, fluorescence-tagged variant 2 localized in the cytoplasm as well as centrosomes.
However, at the beginning of mitosis, the fluorescence appeared as a pair of bright nuclear foci that followed centrosome localization throughout mitosis, while maintaining diffuse cytoplasmic labeling.
Endogenous variant 2 in HeLa cells showed a similar staining pattern.
Centrosomal localization was independent of microtubules.
Applications:Suitable for use in Western Blot, Immunohistochemistry, ELISA
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phophatase (AP).
Specificity：Human
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：SCYL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human SCYL1.
