JMJD2B is histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a role in histone code.
This protein does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'.
It is only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D.
It is demethylation of Lys residue generates formaldehyde and succinate.
Applications:Suitable for use in Dot Blot, ELISA
Recommended Dilution:ELISA: 1:1,000Dot blot 1:500
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phophatase (AP).
Specificity：Human
Isotype：IgG
Calc Applications Abbrev：DB E
Calc Crossreactivity：Hu
Immunogen：Phospho-JMJD2B-S622 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 609~638 amino acids surrounding S622 of human JMJD2B.
