External and internal DNA-damaging agents continually threaten the integrity of genetic material in cells.
Although a variety of repair mechanisms exist to remove the resulting lesions, some lesions escape repair and block the replication machinery.
Members of the Y family of DNA polymerases, such as POLK, permit the continuity of the replication fork by allowing replication through such DNA lesions.
Each Y family polymerase has a unique DNA-damage bypass and fidelity profile.
POLK is specialized for the extension step of lesion bypass (summary by Lone et al., 2007 [PubMed 17317631]).
Applications:Suitable for use in Western Blot, ELISA
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phophatase (AP).
Specificity：Human
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：POLK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 531-560 amino acids from the Central region of human POLK.
