84-1518-46　［取扱停止］CLP1, CT (CLP1, HEAB, Polyribonucleotide 5'-hydroxyl-kinase Clp1, Polynucleotide kinase Clp1, Pre-mRNA cleavage complex II protein Clp1) (AP) 200ul　034008-AP

特徴

• Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids.
• dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component.
• Appears to have roles in both tRNA splicing and mRNA 3'-end formation.
• Component of the tRNA splicing endonuclease complex.
• Phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA.
• Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation.
• Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC).
• However endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.
• Applications:Suitable for use in Western Blot, ELISA
• Recommended Dilution:ELISA: 1:1,000Western Blot: 1:100-500
• Storage and Stability:Store product at 4°C.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.