Pkmyt1 acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins.
Mediates phosphorylation of CDK1 predominantly on 'Thr-14'.
Also involved in Golgi fragmentation.
May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect.
May be a downstream target of Notch signaling pathway during eye development (By similarity).
Applications:Suitable for use in Western Blot, ELISA
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phophatase (AP).
Specificity：Human, mouse
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Mouse Pkmyt1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 113-143 amino acids from the N-terminal region of Mouse Pkmyt1.
