Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA.
Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles.
Methylates SUPT5H.
Mono-and dimethylates arginine residues of myelin basic protein (MBP) in vitro.
Plays a role in the assembly of snRNP core particles.
May play a role in cytokine-activated transduction pathways.
Negatively regulates cyclin E1 promoter activity and cellular proliferation.
May regulate the SUPT5H transcriptional elongation properties.
May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement.
Methylates histone H2A and H4 'Arg-3' during germ cell development.
Methylates histone H3 'Arg-8', which may repress transcription.
Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.
Methylates RPS10.
Applications:Suitable for use in Western Blot, Immunohistochemistry, ELISA
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phophatase (AP).
Specificity：Human
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：PRMT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-104 amino acids from the N-terminal region of human PRMT5.
