84-1475-26　［取扱停止］AKT1 (Thr308) (AKT1, PKB, RAC, RAC-alpha serine/threonine-protein kinase, Protein kinase B, Protein kinase B alpha, Proto-oncogene c-Akt, RAC-PK-alpha) (AP) 200ul　031750-AP

特徴

• The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts.
• AKT1 and the related AKT2 are activated by platelet-derived growth factor.
• The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1.
• It was shown that the activation occurs through phosphatidylinositol 3-kinase.
• In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival.
• Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.
• Multiple alternatively spliced transcript variants have been found for this gene.
• [provided by RefSeq].
• Applications:Suitable for use in Western Blot, Immunohistochemistry, ELISA
• Recommended Dilution:ELISA: 1:1,000Western Blot: 1:100-500Immunohistochemistry: 1:50-1001:10-50
• Storage and Stability:Store product at 4°C.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.