cAMP is a signaling molecule important for a variety of cellular functions.
cAMP exerts its effects by activating the cAMP-dependent protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins.
The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits.
cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits.
Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans.
PRKACB is a member of the Ser/Thr protein kinase family and is a catalytic subunit of AMPK.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:1,000 Western Blot: 1:100-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity.
Light sensitive.
Aliquots are stable for at least 6 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A/G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with Alkaline Phosphatase
Specificity：Recognizes human PKA C-beta
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 17-47 in the N-terminal region of human PKA C-beta, UniProt Accession #P22694.
