84-1465-03　［取扱停止］Hexokinase 2, NT (Hexokinase-2, Hexokinase Type II, HK II, HKII, HK2, HXK2, DKFZp686M1669, Muscle Form Hexokinase) (AP) 200ul　H2035-30N-AP

特徴

• In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III, and IV.
• Hexokinase is an allosteric enzyme inhibited by its product GLC-6-P.
• Hexokinase activity is involved in the first step in several metabolic pathways.
• HK3 is bound to the outer mitochondrial membrane.
• Its hydrophobic N-terminal sequence may be involved in membrane bindng.
• It is the predominant hexokinase isozyme expressed in insuline-responsive tissues such as skeletal muscle.
• The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other.
• The catalytic activity is associated with the C-terminus while regulatory function is associated wiht the N-terminus.
• Although found in NIDDM patients, genetic variations of HK2 do not contribute to the disease.
• Applications:Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:1,000Western Blot: 1:100-1:500Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.