AKT2 is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains.
Furthermore, AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors.
Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers.
AKT2 is a general protein kinase capable of phophorylating several known proteins.
Applications:Suitable for use in ELISA and Dot Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:1,000 Dot Blot: 1:100-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phophatase (AP).
Specificity：Recognizes human AKT2 when phosphorylated at Ser474.
Isotype：IgG
Calc Applications Abbrev：DB E
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated synthetic phosphopeptide mapping to a fragment of residues within amino acids surrounding S474 of human AKT2, UniProt Accession #P31751.
