One of the major roles of the sulfotransferases (ST) in the metabolism of drugs and endogenous compounds is the conversion of these substances into more hydrophilic water-soluble sulfate conjugates that can be easily excreted.
Sulfation may also play a regulatory role for many endogenous compounds, such as steroids and neurotransmitters, by altering the biologic properties of these compounds.
Otterness et al.
(1992), Kong et al.
(1992), and Comer et al.
(1993) reported the cloning of cDNAs encoding liver dehydroepiandrosterone (DHEA) sulfotransferase.
The predicted protein has 285 amino acids.
Although Northern blot analysis of human liver RNA detected transcripts of 3 different sizes, Southern blot analysis of human DNA suggested that only 1 gene is present in the genome.
This gene has an important role in the sulfation of both bile acids and steroids in the liver and adrenals.
The human adrenal form of this enzyme is physically, immunologically, and kinetically similar, perhaps identical, to the liver form.
Dehydroepiandrosterone sulfate is quantitatively one of the major steroids secreted from the adrenal cortex.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:1,000 Western Blot: 1:100-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phophatase (AP).
Specificity：Recognizes human SULT2A. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide selected from the C-terminal region of human SULT2A (KLH).
