Stimulation of cells with cytokines initiates a signal transduction cascade involving cytokine receptors, Janus kinases (JAKs) and signal transducers and activators of transcription (STATs).
STAM for 'signal-transducing adaptor molecule, induced after stimulation of cells with cytokine IL2, is a component of signal transduction downstream of JAK3.1 Human STAM cDNA cloned from a T-cell cDNA library encodes a 540aa protein precipitated by anti-phosphotyrosine.
Northern blot analysis indicates that STAM is expressed as a 2.9-kb message in a wide variety of tissue and cell types.
The STAM sequence contains a Src-homology 3 (SH3) domain and an immunoreceptor tyrosine-based activation motif (ITAM).
It has been suggested that STAM acts as an adaptor molecule in signal transduction pathways from cytokine receptors.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:Immunohistochemistry: Formalin-fixed, paraffin-embedded sectionsOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A/G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phophatase (AP).
Specificity：Recognizes human STAM.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 326-356 in the C-terminal region of human STAM. Species sequence homology: mouse
