84-1452-68 ［取扱停止］UBC9, NT (SUMO-conjugating Enzyme UBC9, SUMO-protein Ligase, Ubiquitin-conjugating Enzyme E2 I, Ubiquitin-protein Ligase I, Ubiquitin Carrier Protein I, Ubiquitin Carrier Protein 9, p18, UBE2I, UBC9, UBCE9) (AP) 200ul U0880-03D-AP
84-1452-68 ［取扱停止］UBC9, NT (SUMO-conjugating Enzyme UBC9, SUMO-protein Ligase, Ubiquitin-conjugating Enzyme E2 I, Ubiquitin-protein Ligase I, Ubiquitin Carrier Protein I, Ubiquitin Carrier Protein 9, p18, UBE2I, UBC9, UBCE9) (AP) 200ul U0880-03D-AP
UBE2I (Ubc9) is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins.
SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation.
This E2 is unusual in that it interacts directly with protein substrates that are modified by sumoylation, and may play a role in substrate recognition.
UBE2I can mediate the conjugation of SUMO-1 to a variety of proteins including RanGAP1, IκBα, and PML without the requirement of an E3 ligase.
UBE2I is essential for nuclear architecture and chromosome segregation.
Applications:Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:1,000Western Blot: 1:100-1:500Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A/G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phophatase (AP).
Specificity：Recognizes human UBC9.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1-30 in the N-terminal region of human UBC19, UniProt Accession #P63279.
