84-1452-36 [取扱停止]Rho-associated Protein Kinase 2 (ROCK2) 100ug 138958
特徴
Rho-associated protein kinase 2 (ROCK2) is a serine/threonine kinase that phosphorylates many important signalling proteins involved in the regulation of cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions complexes as well as malignant cell transformation, tumor invasion and metastasis.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (FFPE): 0.5-1ug/ml.
Heat mediated antigen retrieval is required
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ug
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a lyophilized powder in PBS, 5% BSA, 0.05% Thimerosal, 0.05% sodium azide. Reconstitute with 100ul sterile ddH2O.
Specificity:Recognizes human Rho-associated protein kinase 2, at 160kD. Species Crossreactivity: Rat.
Isotype:IgG
Calc Applications Abbrev:IHC WB
Calc Crossreactivity:Hu Rt
Immunogen:Synthetic peptide corresponding to aa35-51 from human Rho-associated protein kinase 2. Species sequence homology: mouse
Shelf Life:1year
EU Commodity Code:30021010
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