84-1452-31　［取扱停止］Growth Associated Protein 43 (GAP-43) 100ug　138841

特徴

• Growth-associated protein 43 (GAP-43) is expressed by developing and regenerating neurons, and to a lesser extent, reactive glial cells.
• It is also a major component of neuronal growth cones.
• GAP-43 binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence.
• Phosphorylation of GAP-43 by a protein Kinase C is correlated with certain forms of synaptic plasticity.
• GAP-43 is used to specifically label injured neurons and to score neuronal regeneration.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml Immunohistochemistry (FFPE): 0.5-1ug/ml.
• Heat mediated antigen retrieval is recommended
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.