Siglecs (sialic acid binding Ig-like lectins) are Itype lectins that belong to the immunoglobulin superfamily.
They are characterized by a V-type-Iglike domain which mediates sialic acid binding, followed by varying numbers of C2-type Iglike domains.
Mouse Siglec-G, the apparent ortholog of human Siglec-10, is a 110-120kD, 688 amino acid (aa) type I transmembrane protein mainly expressed on mouse B1-type B cells.
It controls B1 cell survival, selection, expansion and calcium signaling by negatively regulating B cell receptor signals.
A potentially secreted 269aa variant diverges after the first two Ig-like domains.
Mouse Siglec-G shares ~63% aa sequence identity with human Siglec-10 within the extracellular domain.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-200Western Blot: 1:100-400Immunocytochemistry: 1:100-500Immunohistochemistry: 1:100-500 (frozen)Immunohistochemistry: 1:50-200 (paraffin sections) Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human Sialic Acid Binding Ig Like Lectin 10 (SIGLEC10)
