Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-200Western Blot: 1:100-400Immunocytochemistry: 1:100-500Immunohistochemistry: 1:100-500 (frozen)Immunohistochemistry: 1:50-200 (paraffin sections) Optimal dilutions to be determined by the researcher.
AA Sequence:MGHHHHHHSGSEF-MSELEKAMVA LIDVFHQYSG REGDKHKLKK SELKELINNE LSHFLEEIKE QEVVDKVMET LDNDGDGECD FQEFMAFVAM VTTACHEFFE HE
Positive Control (Supplied):142203A: Suppled as a recombinant protein S100B (Met1~Glu92) in 100mM Tris, pH 8.8, 2% SDS, 200mM sodium chloride, BPB 0.01%, 0.02% sodium azide, 50% glycerol,DAB: 10ul/well ECL: 5ul/wellStore at -20°C.
Stable for 6 months after receipt.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：guinea pig
Source Antibody：rabbit
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes rabbit S100 Calcium Binding Protein B (S100B)
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：E. coli-expressed recombinant rabbit S100B with N-terminal His-tag
