Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:200Western Blot: 1:50-1:400Immunocytochemistry: 1:50-1:500Immunohistochemistry(frozen sections): 1:50-1:500Immunohistochemistry(paraffin sections): 1:10-1:100Optimal dilutions to be determined by the researcher.
Positive Control (Supplied):141725A: Suppled as a recombinant protein OSM (Asn25~Leu115) in 100mM Tris, pH 8.8, 2% SDS, 200mM sodium chloride, 0.01% BPB, 0.02% sodium azide, 50% glycerol
DAB: 10ul/well ECL: 5ul/well Store at -20°C.
Stable for 6 months after receipt.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:200ul
Host:rabbit
Source Antibody:mouse
Grade:Affinity Purified
Purity:Purified by affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide, 50% glycerol.