84-1440-59 Nephronectin (NPNT, EGFL6L, POEM) 200ul 141602
特徴
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:100-1:200Western Blot: 1:100-1:400Immunocytochemistry: 1:100-1:500Immunohistochemistry (frozen): 1:100-1:500Immunohistochemistry (Paraffin): 1:50-1:200Optimal dilutions to be determined by the researcher.
Amino Acid Sequence:MGHHHHHHSGSEF- LVH SCNFDHGLCG WIREKDNDLH WEPIRDPAGG QYLTVSAAKA PGGKAARLVL PLGRLMHSGD LCLSFRHKVT GLHSGTLQVF VRKHGAHGAA LWGRNGGHGW RQTQITLRGA DIKSVVFKGE KRRGHTGEIG LDDVSLKKGH CSEER
Positive Control Included:141602A: Nephronectin, Positive ControlSuppled as a recombinant protein in 100mM Tris, pH 8.8, 2% SDS, 200mM sodium chloride, 0.01% BPB, 0.02% sodium azide, 50% glycerol
DAB: 10ul/wellECL: 5ul/wellStore at -20°C.
Stable for 6 months after receipt.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:200ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide, 50% glycerol.
Specificity:Recognizes human Nephronectin (NPNT)
Isotype:IgG
Calc Applications Abbrev:E IC IHC WB
Calc Crossreactivity:Hu
Immunogen:Recombinant protein coresponding to aa418-565 of human Nephronectin with N-terminal His-Tag expressed in E. coli (Q6UXI9).
Shelf Life:6months
Swiss Prot Number:Q6UXI9
EU Commodity Code:30021010
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