84-1435-97　Keratin 10 (KRT10, K10, CK10, KPP, Cytokeratin 10, Epidermolytic Hyperkeratosis, Keratosis Palmaris Et Plantaris) 200ul　141121

特徴

• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilutions:ELISA: 1:100-1:200Western Blot: 1:50-1:400Immunohistochemistry (frozen): 1:50-1:500Immunohistochemistry (Paraffin): 1:10-1:100Immunocytochemistry: 1:50-1:500 Optimal dilutions to be determined by the researcher.
• Amino Acid Sequence:MGHHHHHHSGSEF-LNDRLASY LDKVRALEES NYELEGKIKE WYEKHGNSHQ GEPRDYSKYY KTIDDLKNQI LNLTTDNANI LLQIDNARLA ADDFRLKYEN EVALRQSVEA DINGLRRVLD ELTLTKADLE MQIESLTEEL AYLKKNHEEE MKDLRNVSTG DVNVEMNAAP GVDLTQLLNN MRSQYEQLAE QNRKDAEAWF NEKSKELTTE IDNNIEQISS YKSEITELRR NVQALEIELQ SQLALKQSLE ASLAETEGRY CVQLSQIQAQ ISALEEQLQQ IRAETECQNT EYQQLLDIKI RLENEIQTYR SLLEGE
• Positive Control (Supplied):141121A: Suppled as a recombinant protein (aa153-456) in loading buffer (100mM Tris, pH 8.8, 2% SDS, 200mM sodium chloride, 0.01% BPB, 0.02% sodium azide, 50% glycerol)
• DAB: 10ul/wellECL: 5ul/wellStore at -20°C.
• Stable for 6 months after receipt.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.